Thymosin Beta-4 (commonly known as TB-500) is a naturally derived repair-support peptide used in advanced wellness and performance programs to help the body cope with tissue stress, micro-injury and recovery demands. It is based on a peptide sequence found widely in the body, particularly in cells that are involved in repair and regeneration. In practice, TB-500 is chosen for its potential to support healing processes in muscle, fascia, tendons and other soft tissues, as well as for its broader role in cell migration, tissue remodelling and resilience.

Clients who use TB-500 under clinical supervision are usually dealing with high physical demands, ongoing training loads or a history of soft-tissue niggles and slow recovery. Rather than being a painkiller or a local anti-inflammatory injection, TB-500 is positioned as a systemic support tool that may help enhance the body’s own capacity to repair and remodel tissues over time. When paired with good rehab, strength work, mobility programming and adequate nutrition (especially protein and collagen-building nutrients), it can become one part of a more comprehensive strategy to keep people moving, training and functioning at a higher level.

It is not a substitute for proper diagnosis, imaging, surgery when required, deloading or structured rehabilitation. TB-500 should only be used under the guidance of a clinician who understands its role, risks and limitations, and who can ensure that any underlying structural problems, red-flag symptoms or medical issues are properly assessed and managed.

When used as directed and as part of a complete treatment and rehab plan, clients commonly use Thymosin Beta-4 (TB-500) to help support:

Soft-tissue healing and repair

TB-500 is often included in programs for muscle, tendon, fascia and ligament support, with the aim of helping the body repair micro-injuries and accumulated strain more effectively.

Recovery from training and physical load

Active people and athletes may use TB-500 to support recovery between sessions and training blocks, alongside proper programming, deloads and recovery strategies.

Mobility and function

By supporting soft-tissue quality and repair, TB-500 can be part of a plan to improve comfort with movement and maintain or regain functional range of motion.

Healthy-ageing of joints and connective tissues

TB-500 is sometimes used in longevity or active-ageing protocols to help support the tissues that keep people moving well as they get older.

Inflammation and fibrosis modulation (TB4 Frag aa 1–4)

The aa 1–4 fragment has been studied for its anti-inflammatory and anti-fibrotic signalling, and is used clinically to support more balanced tissue responses in recovery and healthy-ageing contexts. 

Targeted repair, angiogenesis and hair / follicle support (TB4 Frag aa 17–23)

The aa 17–23 fragment (TB-500 region) is associated with enhanced cell migration, wound repair, vascular support and hair-follicle regeneration in research settings, and is therefore included where deeper tissue repair and regenerative goals are a focus.

*Individual results vary. TB-500 is not a stand-alone treatment for musculoskeletal injury, arthritis or any other condition, and should always be used alongside appropriate medical care, imaging and rehabilitation where indicated.

Active Ingredient

Thymosin Beta-4 (TB-500) peptide – 3000 mcg/mL

Presentation – Injectable Vial

Strength: 3000 mcg/mL

Volume: 5 mL multidose vial

Total peptide content per vial: 15,000 mcg (15 mg)

Excipients

Sterile diluent (e.g., bacteriostatic saline) and standard compounding excipients as specified by the partner pharmacy (may include buffering agents and preservatives).

Presentation – Oral capsules

Strength per capsule:
TB4 Frag (aa 1–4): 500 mcg
TB4 Frag (aa 17–23): 250 mcg

Pack size: 

60 capsules

Protocol & Standard Dosing

Standard Protocol

Dose:

0.10–0.20 mL subcutaneous (SC) nightly

Frequency:

Once daily

Regenerative Protocol

Dose:

0.10 mL SC twice daily (BD – morning and evening)

Frequency: 

Daily

At a concentration of 3000 mcg/mL:

0.10 mL ≈ 300 mcg

0.20 mL ≈ 600 mcg

Your clinician will decide which protocol (standard vs regenerative) is suitable based on your presentation, goals and overall health.

Route: 

Subcutaneous injection into fatty tissue (e.g. lower abdomen, outer thigh) using a fine insulin syringe.

Timing:

Standard: once nightly (e.g. in the evening, same time each day).

Regenerative: morning and evening, roughly 12 hours apart, or as directed.

Technique:

 Clean the area, pinch a fold of fat, inject as instructed by your nurse or clinician, rotate injection sites and use aseptic technique at all times.

Capsules

Timing: 

Often taken once or twice daily, with water, at the same times each day to maintain consistent levels, as directed.

With or without food: Your practitioner may recommend taking with food if you are prone to digestive sensitivity.

Administration Instructions

Swallow capsules whole with water; do not open, crush or chew unless your clinician advises otherwise.

Do not exceed the prescribed daily number of capsules, and do not share this medication with others.

Do not change your dose, frequency or protocol without discussing this with your prescribing clinician.

Side Effects, Reactions & Medical Contraindications

Use is generally not recommended in:

• Known allergy or hypersensitivity to Thymosin Beta-4 or any component of the vial
• Pregnancy or breastfeeding (insufficient safety data)
• Active malignancy or history of cancer unless cleared and supervised by an appropriate specialist, given the relationship between repair signalling and cell growth
• Severe uncontrolled systemic illness or infection without clear clinical oversight
• People with complex autoimmune or connective tissue disorders unless coordinated with a relevant specialist
• A thorough medical history, medication list and, where appropriate, imaging and diagnosis should be reviewed before TB-500 is prescribed.

Common Side Effects and Adverse Reactions

• Most side effects reported with TB-500 are mild and may settle as the body adjusts. These can include:
• Injection-site reactions – redness, itching, mild swelling or small bruises
• Transient fatigue or “post-injection heaviness”, particularly early in a course
• Headache or light-headedness in some individuals
• Occasionally mild digestive upset such as nausea
• If side effects are persistent, troublesome or worrying, clients should contact their healthcare provider so dose, timing or ongoing suitability can be reviewed.

Reactions Requiring Medical Attention – Stop injections and seek medical attention (or emergency care if severe) if you notice:

• Signs of a serious allergic reaction – widespread rash or hives, swelling of the face, lips or tongue, difficulty breathing or swallowing
• Severe or rapidly worsening pain, swelling, redness or heat at the injection site which may signal an infection
• Any new or rapidly worsening symptom that feels serious, unusual or concerning, especially in those with complex medical histories

Safety Note

• Thymosin Beta-4 (TB-500) is a tissue-repair–oriented peptide, not a simple over-the-counter supplement. Because it interacts with signalling pathways involved in cell movement, repair and remodelling, it should:
• Only be used under the guidance of a qualified clinician
• Be integrated into a broader plan that includes accurate diagnosis, appropriate imaging, manual therapy or rehabilitation, exercise prescription and load management
• Be understood as supportive, not curative – it can help the body’s repair mechanisms, but it does not replace structured rehab, surgery when required or other medical treatment
• Informed consent is important so clients understand what TB-500 can realistically offer, what is still uncertain in the research, and the importance of ongoing monitoring and review.

Safety Note: Long‑term data is limited. Use should always be monitored by a qualified clinician, with regular reviews and ongoing medical supervision.  Informed consent is essential.